22 Dec 2015 Five crystal structures of L. kefir KRED variants were obtained, four of them in the apo state (WT, J Am Chem Soc 130(46):15361–15373.

式（I）（式中、Q1はジヒドロインデニルアミノ基等を示す。）の化合物の製造方法、ならびに中間体の製造方法を提供する。

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The better pH for KRED-130 results between 6.5 and 8, being 7 the best value and Km was 30,7 mM, while for ketoreducase from U52011207172 better pH is between 5 and 6, with best results at pH 5,5 and Km 3,4 mM. In a 2.0 mL Eppendorf tube, KRED (2 mg), 3 (27 mM), DMSO (55 μL) and i‐PrOH (165 μL) were added to 900 μL of 125 mM KH 2 PO 4 buffer (1.25 mM MgSO 4, 1 mM NADP +) pH 7.0 or 900 μL of 250 mM KH 2 PO 4 buffer (2 mM MgSO 4, 1.1 mM NADP +, 80 mM d ‐glucose, 10 U/mL glucose dehydrogenase) pH 7.0 in the case of KRED‐101, KRED‐119, KRED‐130, KRED‐NADH‐101 and KRED‐NADH‐110. In 2016, Clayden and co-workers developed a biocatalytic reduction for the atropo-enantioselective synthesis of axially chiral heterobiaryl N-oxides via dynamic kinetic resolution . 44 Particularly, a ketoreductase (KRED 130) was used for the reduction of a configurationally unstable heterobiaryl ketone under mild conditions, while glucose is oxidized to gluconic acid. After screening a commercial kit of reductases, the KRED-130 gave access to the enantiopure (R)-(-ketal-β-hydroxy ester in 86% isolated yield in a 32 mmol scale (Scheme 49). Scheme 49. Bioreduction of a (-ketal-β-keto ester for the synthesis of statins.

In one aspect of, the enzyme is selected from CRED A131, CRED A251, KRED-NADH 101, KRED-NADH 102, KRED-NADH 112, KRED-NADH 126, ES-KRED-121, ES-KRED-128, ES-KRED-130, ES-KRED-142, ES-KRED-169, or ES-KRED-171. In a more specific aspect, the enzyme is selected from CRED A131, CRED A251, and KRED-NADH 101.

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Komisja Nadzoru Budowl Korony 210.1. [1816-1817] 1818-1832 [1833-1839], 1854 4 mb (180 j.a.) 1 1818-1831 Akta organizacji Komisji Nadzoru Budowl Korony, z 1818 roku, vol. I poszyt, s. 53 pol. 1 2 1820-1821 Protokół posiedzeń Komisji Nadzoru Budowl Korony, 1820 r. poszyt, s. 19 pol. brak 3 1818 [Protokół posiedzeń Komisji Nadzoru Budowl Korony] poszyt, s. 11 pol. brak 4 1820

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